Two single shot obtained using the IS DMK31AF03 on Horse Head Nebula in Orion with a H-alfa filter (6nm) at 315" and 632":

 

Image obtained from processing of 6 frames at 315" and 3 at 632" (Sigma-median) just the offset subtractred:

The offset frame at 4" to evidentiate the hotpixels:

The dark frame at 315" to evidentiate the termal noise: